ELISA/assay

Rat 5-hydroxy tryptamine (5-ht) ELISA Kit

MBS266539

48-Strip-Wells

290 USD

ELISA Kit

Rat 5-hydroxy tryptamine (5-ht) ELISA Kit

5-hydroxy tryptamine (5-ht)

5-hydroxytryptamine receptor 1A; 5-hydroxytryptamine (Serotonin) receptor 1A; 5-hydroxytryptamine receptor 1A; 5-HT1a receptor; guanine nucleotide-binding regulatory protein-coupled receptor; 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled; 5-hydroxytryptamine (Serotonin) receptor 1A; 5-hydroxytryptamine receptor 1A

5-ht

HTR1A; 5HT1A; G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1; HTR1A; hCG_16836

Q5ZGX3_HUMAN

Rat

422

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant 5-ht concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat 5-ht monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

53748104

CAH03197.1

Q5ZGX3

46,107 Da

Amine Ligand-binding Receptors Pathway (106363); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Monoamine GPCRs Pathway (198861); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); SIDS Susceptibility Pathways (198901)

This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers. [provided by RefSeq, Jun 2012]

5-HT(1A): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family. 5- hydroxytryptamine receptor subfamily. HTR1A sub-subfamily. Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1. Chromosomal Location of Human Ortholog: 5q11.2-q13. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: protein binding; serotonin receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; cell proliferation; vasoconstriction; behavioral fear response; serotonin receptor, adenylate cyclase inhibiting pathway; regulation of dopamine metabolic process; positive regulation of cell proliferation; regulation of serotonin secretion; regulation of hormone secretion; serotonin receptor signaling pathway; serotonin metabolic process; regulation of behavior. Disease: Periodic Fever, Menstrual Cycle-dependent

48-Strip-Wells

290 USD

96-Strip-Wells

450