ELISA/assay

Rat Prostaglandin F2alpha (PGF2alpha) ELISA Kit

MBS266512

48-Strip-Wells

290 USD

ELISA Kit

Rat Prostaglandin F2alpha (PGF2alpha) ELISA Kit

Prostaglandin F2alpha (PGF2alpha)

Rat Prostaglandin F2a (PGF2a)  ELISA Kit

prostaglandin F2alpha receptor, partial; Prostaglandin F2-alpha receptor; prostaglandin F2-alpha receptor; PGF receptor; PGF2 alpha receptor; PGF2-alpha receptor; prostanoid FP receptor; prostaglandin F2 alpha receptor; prostaglandin receptor (2-alpha); prostaglandin F receptor (FP); Prostanoid FP receptor

PGF2alpha

PTGFR; PTGFR; FP; PGF receptor; PGF2-alpha receptor

PF2R_HUMAN

Rat

266

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PGF2? concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat PGF2? monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

7619989

BAA94756.1

30,766 Da

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Eicosanoid Ligand-binding Receptors Pathway (106370); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); GPCRs, Other Pathway (198765); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295)

The protein encoded by this gene is member of the G-protein coupled receptor family. This protein is a receptor for prostaglandin F2-alpha (PGF2-alpha), which is known to be a potent luteolytic agent, and may also be involved in modulating intraocular pressure and smooth muscle contraction in uterus. Knockout studies in mice suggest that the interaction of PGF2-alpha with this receptor may initiate parturition in ovarian luteal cells and thus induce luteolysis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

PTGFR: Receptor for prostaglandin F2-alpha (PGF2-alpha). The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. Initiates luteolysis in the corpus luteum. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR. Chromosomal Location of Human Ortholog: 1p31.1. Cellular Component: integral to plasma membrane; cytoplasm; extracellular region; plasma membrane. Molecular Function: prostaglandin F receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of cell proliferation; response to lipopolysaccharide; parturition; negative regulation of apoptosis

48-Strip-Wells

290 USD

96-Strip-Wells

450