ELISA/assay

Human Cytochrome C (Cyt-C) ELISA Kit

MBS266489

48-Strip-Wells

275 USD

ELISA Kit

Human Cytochrome C (Cyt-C) ELISA Kit

Cytochrome C (Cyt-C)

cytochrome c; Cytochrome c; cytochrome c; cytochrome c, somatic

Cyt-C

CYCS; CYCS; CYC; HCS; THC4; CYC

CYC_HUMAN

Human

105

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant Cyt-C concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human Cyt-C monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

11128019

NP_061820.1

NM_018947.5

11,749 Da

AGE/RAGE Pathway (698754); Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (105672); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470)

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

CYCS: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family. Protein type: Mitochondrial; Protein phosphatase, Ser/Thr (non-receptor); Oxidoreductase; Apoptosis. Chromosomal Location of Human Ortholog: 7p15.3. Cellular Component: protein phosphatase type 2A complex; mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol; nucleus. Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; protein binding; iron ion binding; heme binding; protein serine/threonine phosphatase activity. Biological Process: cellular metabolic process; mitochondrion organization and biogenesis; response to reactive oxygen species; dephosphorylation; apoptosis; organelle organization and biogenesis; cellular respiration; mitochondrial electron transport, cytochrome c to oxygen; caspase activation via cytochrome c; DNA fragmentation during apoptosis; transmembrane transport; mitochondrial electron transport, ubiquinol to cytochrome c. Disease: Thrombocytopenia 4

48-Strip-Wells

275 USD

96-Strip-Wells

420