ELISA/assay

Porcine Insulin Like Growth Factor 1 (IGF-1) ELISA Kit

MBS266488

48-Strip-Wells

275 USD

ELISA Kit

Porcine Insulin Like Growth Factor 1 (IGF-1) ELISA Kit

[Insulin Like Growth Factor 1 (IGF-1)]

[insulin-like growth factor I preproprotein; Insulin-like growth factor I; insulin-like growth factor I; somatomedin; prepro-IGF-I; insulin growth factor-1; insulin like growth factor 1; insulin-like growth factor-1; insulin-like growth factor I variant 1; insulin-like growth factor I (somatomedin C); class 1 insulin-like growth factor I preproprotein; insulin-like growth factor 1 (somatomedin C); Somatomedin]

[IGF-1]

[IGF1; IGF1; IGF-1; IGF-I; IGF-I]

IGF1_BOVIN

Porcine

154

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Porcine Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Ifn-gamma Concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine IFN-gamma monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

118150782

NP_001071296.1

NM_001077828.1

17,066 Da

Aldosterone-regulated Sodium Reabsorption Pathway (130625); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Androgen Receptor Signaling Pathway (198553); Apoptosis Pathway (198572); Dilated Cardiomyopathy Pathway (121480); Dilated Cardiomyopathy Pathway (121285); Focal Adhesion Pathway (84306); Focal Adhesion Pathway (478); Glioma Pathway (84345); Glioma Pathway (522)

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 ().

48-Strip-Wells

275 USD

96-Strip-Wells

435

5x96-Strip-Wells

1755

10x96-Strip-Wells

3150