catalog number :
MBS266431
products type :
ELISA Kit
products full name :
Human Pleiotrophin (PTN) ELISA Kit
products short name :
Pleiotrophin (PTN)
other names :
pleiotrophin; Pleiotrophin; pleiotrophin; HBBM; OSF-1; HB-GAM; HBGF-8; HBNF-1; osteoblast-specific factor 1; heparin-binding brain mitogen; heparin binding growth factor 8; heparin-binding growth factor 8; heparin affin regulatory protein; neurite growth-promoting factor 1; heparin-binding growth-associated molecule; heparin-binding neurite outgrowth-promoting factor 1; pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1); pleiotrophin; Heparin-binding brain mitogen; HBBM; Heparin-binding growth factor 8; HBGF-8; Heparin-binding growth-associated molecule; HB-GAM; Heparin-binding neurite outgrowth-promoting factor 1; HBNF-1; Osteoblast-specific factor 1; OSF-1
other gene names :
PTN; PTN; HARP; HBNF; HBGF8; NEGF1; HBNF1; NEGF1; PTN; HBBM; HBGF-8; HB-GAM; HBNF-1; OSF-1
uniprot entry name :
PTN_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant PTN concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PTN monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_002816.1
ncbi gb acc num :
NM_002825.5
ncbi mol weight :
18,942 Da
ncbi pathways :
Syndecan-3-mediated Signaling Events Pathway (137952)
uniprot summary :
PTN: Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti- apoptotic signaling of PTN and regulation of cell proliferation. Belongs to the pleiotrophin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q33. Cellular Component: extracellular space; endoplasmic reticulum. Molecular Function: heparin binding; growth factor activity; protein phosphatase inhibitor activity. Biological Process: nervous system development; positive regulation of cell division; positive regulation of cell proliferation; negative regulation of catalytic activity; learning; transmembrane receptor protein tyrosine phosphatase signaling pathway; bone mineralization