Guinea pig cartilage oligomeric matrix protein (COMP) ELISA Kit | MyBioSource MBS266330 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Guinea pig cartilage oligomeric matrix protein (COMP) ELISA Kit

catalog :

MBS266330

quantity :

48-Strip-Wells

price :

315 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS266330

products type :

ELISA Kit

products full name :

Guinea pig cartilage oligomeric matrix protein (COMP) ELISA Kit

products short name :

cartilage oligomeric matrix protein (COMP)

other names :

cartilage oligomeric matrix protein; Cartilage oligomeric matrix protein; cartilage oligomeric matrix protein; TSP5; thrombospondin-5; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein; Thrombospondin-5; TSP5

products gene name :

COMP

other gene names :

COMP; COMP; MED; EDM1; EPD1; PSACH; THBS5; COMP; TSP5

uniprot entry name :

COMP_HUMAN

reactivity :

Guinea Pig

sequence length :

757

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Guinea pig serum, plasma or cell culture supernatant and organizations in the natural and recombinant COMP concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: Up to 0.05 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Guinea pig COMP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

40217843

ncbi acc num :

NP_000086.2

ncbi gb acc num :

NM_000095.2

ncbi mol weight :

77,214 Da

ncbi pathways :

ECM Proteoglycans Pathway (833812); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Malaria Pathway (152665); Malaria Pathway (152657); PI3K-Akt Signaling Pathway (692234)

ncbi summary :

The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2008]

uniprot summary :

COMP: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. Belongs to the thrombospondin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19p13.1. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region. Molecular Function: collagen binding; heparan sulfate proteoglycan binding; heparin binding; protein binding; protease binding; extracellular matrix structural constituent; calcium ion binding. Biological Process: limb development; organ morphogenesis; extracellular matrix organization and biogenesis; apoptosis; cell adhesion; skeletal development; negative regulation of apoptosis. Disease: Epiphyseal Dysplasia, Multiple, 1; Pseudoachondroplasia

size1 :

48-Strip-Wells

price1 :

315 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage