ELISA/assay

Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

MBS266295

48-Strip-Wells

290 USD

ELISA Kit

Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

Glial Fibrillary Acidic Protein (GFAP)

glial fibrillary acidic protein; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein

GFAP

GFAP; GFAP; GFAP

GFAP_HUMAN

Mouse

432

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant GFAP concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse GFAP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

251802

AAB22581.1

49,508 Da

Neural Crest Differentiation Pathway (672460); Nuclear Signaling By ERBB4 Pathway (530744); Signal Transduction Pathway (477114); Signaling By ERBB4 Pathway (530741); Spinal Cord Injury Pathway (739007)

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: membrane; cytoplasm; intermediate filament; cytosol. Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding. Biological Process: extracellular matrix organization and biogenesis; Bergmann glial cell differentiation; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development. Disease: Alexander Disease

48-Strip-Wells

290 USD

96-Strip-Wells

450