ELISA/assay

Human alpha-Smooth muscle actin (alpha-SMA) ELISA Kit

MBS266274

48-Strip-Wells

275 USD

ELISA Kit

Human alpha-Smooth muscle actin (alpha-SMA) ELISA Kit

alpha-Smooth muscle actin (alpha-SMA)

Human a-Smooth muscle actin (a-SMA) ELISA Kit

actin, gamma-enteric smooth muscle isoform 1; Actin, gamma-enteric smooth muscle; actin, gamma-enteric smooth muscle; alpha-actin-3; actin-like protein; actin, gamma 2, smooth muscle, enteric; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin

alpha-SMA

ACTG2; ACTG2; ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG; ACTA3; ACTL3; ACTSG

ACTH_HUMAN

Human

376

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant alpha-SMA concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human alpha-SMA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4501889

NP_001606.1

NM_001615.3

37,083 Da

Muscle Contraction Pathway (106261); Smooth Muscle Contraction Pathway (161025); Vascular Smooth Muscle Contraction Pathway (96530); Vascular Smooth Muscle Contraction Pathway (96866)

Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010]

ACTG2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family. Protein type: Motility/polarity/chemotaxis; Contractile; Cytoskeletal. Chromosomal Location of Human Ortholog: 2p13.1. Cellular Component: extracellular space; cytoskeleton; cytosol. Molecular Function: ATP binding. Biological Process: muscle contraction. Disease: Visceral Myopathy

48-Strip-Wells

275 USD

96-Strip-Wells

420