ELISA/assay

Mouse Stromelysin-3 (ST3) ELISA Kit

MBS266272

48-Strip-Wells

290 USD

ELISA Kit

Mouse Stromelysin-3 (ST3) ELISA Kit

Stromelysin-3 (ST3)

stromelysin-3, partial; Stromelysin-3; stromelysin-3; MMP-11; stromelysin III; matrix metallopeptidase 11 (stromelysin 3); Matrix metalloproteinase-11; MMP-11

ST3

MMP11; MMP11; ST3; SL-3; STMY3; STMY3; SL-3; ST3; MMP-11

MMP11_HUMAN

Mouse

36

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ST3 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse ST3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

987949

CAA59150.1

P24347

54,590 Da

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of Collagen Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Matrix Metalloproteinases Pathway (198900)

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is activated intracellularly by furin within the constitutive secretory pathway. Also in contrast to other MMP's, this enzyme cleaves alpha 1-proteinase inhibitor but weakly degrades structural proteins of the extracellular matrix. [provided by RefSeq, Jul 2008]

MMP11: May play an important role in the progression of epithelial malignancies. Belongs to the peptidase M10A family. Protein type: Secreted, signal peptide; Protease; EC 3.4.24.-; Secreted; Extracellular matrix. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: proteinaceous extracellular matrix; Golgi lumen; extracellular region. Molecular Function: zinc ion binding; metalloendopeptidase activity; calcium ion binding. Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; collagen fibril organization; multicellular organismal development; proteolysis; negative regulation of fat cell differentiation

48-Strip-Wells

290 USD

96-Strip-Wells

450