catalog number :
MBS266250
products type :
ELISA Kit
products full name :
Mouse Dihydrotestosterone (DHT) ELISA Kit
products short name :
Dihydrotestosterone (DHT)
other names :
differentially repressed by testosterone and dihydrotestosterone; Protein NDRG1; N-myc downstream-regulated gene 1 protein; Protein Ndr1
other gene names :
Ndrg1; Ndr1; Ndrl; Tdd5; Protein Ndr1
uniprot entry name :
NDRG1_MOUSE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant DHT concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse DHT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAB58249.1
ncbi mol weight :
43,009 Da
uniprot summary :
NDRG1: a hypoxia-inducible anti-apoptotic protein. Promotes proliferation and correlates with invasion, metastasis and poor prognosis in many cancers. Is involved in growth arrest and cell differentiation during development. A ubiquitous Rab4a effector protein that modulates angiogenesis and is involved in vesicular recycling of E-cadherin. Colocalizes with transferrin during the recycling in cells. NDRG1 knockdown delays the recycling rate of transferrin, while its overexpression increases the rate of transferrin recycling. Interacts with SIRT1/p53 signaling to attenuate hypoxic injury in human trophoblasts. Its expression is induced by an elevation of free intracellular calcium ion caused by nickel ion exposure. Highly expressed in placental membranes, prostate, kidney, small intestine, ovary, peripheral nerves and Schwann cells. Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D). Protein type: Vesicle; Cell development/differentiation. Cellular Component: microtubule cytoskeleton; centrosome; microtubule; cytoskeleton; cell-cell adherens junction; membrane; recycling endosome membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus; myelin sheath. Molecular Function: gamma-tubulin binding; cadherin binding; microtubule binding; Rab GTPase binding. Biological Process: myelin maintenance in the peripheral nervous system; mast cell activation