catalog number :
MBS266237
products type :
ELISA Kit
products full name :
Mouse Tumor Necrosis Factor Alpha (TNF-alpha) ELISA Kit
products short name :
Tumor Necrosis Factor Alpha (TNF-alpha)
products name syn :
Mouse Tumor Necrosis Factor Alpha (TNF-a) ELISA Kit
other names :
tumor necrosis factor alpha; tumor necrosis factor alpha
products gene name :
TNF-alpha
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant TNF-alpha concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse TNF-alpha monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_001121579.1
ncbi gb acc num :
NM_001128107.1