catalog number :
MBS266186
products type :
ELISA Kit
products full name :
Mouse Endostatin (ES) ELISA Kit
products short name :
Endostatin (ES)
other names :
endostatin, partial; Collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; endostatin; procollagen, type XVIII, alpha 1; collagen, type XVIII, alpha 1
other gene names :
Col18a1; Col18a1
uniprot entry name :
COIA1_MOUSE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant ES concentration. Assay Type: Quantitative Sandwich. Sensitivity: Up to 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse ES monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAF69009.1
ncbi mol weight :
134,204 Da
ncbi pathways :
Activation Of Matrix Metalloproteinases Pathway (1001029); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1001018); Collagen Biosynthesis And Modifying Enzymes Pathway (1001017); Collagen Degradation Pathway (1001030); Collagen Formation Pathway (1001016); Degradation Of The Extracellular Matrix Pathway (1001028); Extracellular Matrix Organization Pathway (1001015); Integrin Cell Surface Interactions Pathway (1001031); Laminin Interactions Pathway (1001024); PodNet: Protein-protein Interactions In The Podocyte Pathway (755428)
uniprot summary :
COL18A1: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Defects in COL18A1 are a cause of Knobloch syndrome type 1 (KNO1). An autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Belongs to the multiplexin collagen family. 3 isoforms of the human protein are produced by alternative promoter. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; collagen; extracellular region; basement membrane. Molecular Function: identical protein binding; metal ion binding; structural molecule activity. Biological Process: extracellular matrix organization and biogenesis; positive regulation of cell proliferation; endothelial cell morphogenesis; angiogenesis; cell adhesion; positive regulation of cell migration