Human Acidic Fibroblast Growth Factor (aFGF) ELISA Kit | MyBioSource MBS266169 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Acidic Fibroblast Growth Factor (aFGF) ELISA Kit

catalog :

MBS266169

quantity :

48-Strip-Wells

price :

250 USD

product information

catalog number :

MBS266169

products type :

ELISA Kit

products full name :

Human Acidic Fibroblast Growth Factor (aFGF) ELISA Kit

products short name :

[Acidic Fibroblast Growth Factor (aFGF)]

other names :

[acidic fibroblast growth factor, partial; Fibroblast growth factor 1; fibroblast growth factor 1; heparin-binding growth factor 1; beta-endothelial cell growth factor; endothelial cell growth factor, beta; endothelial cell growth factor, alpha; fibroblast growth factor 1 (acidic); Acidic fibroblast growth factor; aFGF; Endothelial cell growth factor; ECGF; Heparin-binding growth factor 1; HBGF-1]

products gene name :

[aFGF]

other gene names :

[FGF1; FGF1; AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha; FGFA; FGF-1; aFGF; ECGF; HBGF-1]

uniprot entry name :

FGF1_HUMAN

reactivity :

Human

sequence length :

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: human serum, plasma or cell culture supernatant. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/mL

other info2 :

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human aFGF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

456828

ncbi acc num :

AAB29059.1

ncbi mol weight :

6,698 Da

ncbi pathways :

Activated Point Mutants Of FGFR2 Pathway (645281); Adaptive Immune System Pathway (366160); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signal Transduction Pathway (106385); Downstream Signaling Of Activated FGFR Pathway (160957); FGF Signaling Pathway (137989)

ncbi summary :

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]

uniprot summary :

FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine; Motility/polarity/chemotaxis; Cell development/differentiation. Chromosomal Location of Human Ortholog: 5q31. Cellular Component: nucleoplasm; proteinaceous extracellular matrix; extracellular space; extracellular region; nucleolus; cell cortex; cytosol. Molecular Function: heparin binding; protein binding; growth factor activity; Hsp70 protein binding; fibroblast growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; multicellular organismal development; positive regulation of cholesterol biosynthetic process; signal transduction; positive regulation of MAP kinase activity; positive regulation of angiogenesis; induction of an organ; positive regulation of cell division; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of epithelial cell proliferation; lung development; positive regulation of cell migration

size1 :

48-Strip-Wells

price1 :

250 USD

size2 :

96-Strip-Wells

price2 :

365

size3 :

5x96-Strip-Wells

price3 :

1480

size4 :

10x96-Strip-Wells

price4 :

2620