ELISA/assay

Rat Peptidoglycan ELISA Kit

MBS265813

48-Strip-Wells

260 USD

ELISA Kit

Rat Peptidoglycan ELISA Kit

[Peptidoglycan]

Rat

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Peb1 Concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat PEB1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

This gene encodes a peptidoglycan recognition protein, which belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. These proteins are part of the innate immune system and recognize peptidoglycan, a ubiquitous component of bacterial cell walls. This protein binds to murein peptidoglycans of Gram-positive bacteria. [provided by RefSeq, Sep 2011]

PGLYRP3: Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram- negative bacteria, and has bacteriostatic activity towards Gram- negative bacteria. Plays a role in innate immunity. Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: membrane; extracellular region; intracellular. Molecular Function: peptidoglycan receptor activity; peptidoglycan binding; zinc ion binding; N-acetylmuramoyl-L-alanine amidase activity. Biological Process: defense response to Gram-positive bacterium; negative regulation of natural killer cell differentiation during immune response; detection of bacterium; negative regulation of interferon-gamma production; innate immune response; pattern recognition receptor signaling pathway; peptidoglycan catabolic process

48-Strip-Wells

260 USD

96-Strip-Wells

405

5x96-Strip-Wells

1615

10x96-Strip-Wells

2880