product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit
catalog :
MBS265806
quantity :
48-Strip-Wells
price :
290 USD
more info or order :
product information
catalog number :
MBS265806
products type :
ELISA Kit
products full name :
Mouse 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit
products short name :
12-Hydroxyeicosatetraenoic Acid (12-HETE)
other names :
12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor; 12-(S)-HETE receptor; 12-(S)-HETE acid receptor; G-protein coupled receptor 31; probable G-protein coupled receptor 31; bA517H2.2 (G protein-coupled receptor 31); hydroxyeicosatetraenoic (HETE) acid receptor 1; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (HETE) receptor; G protein-coupled receptor 31; G-protein coupled receptor 31
products gene name :
12-HETE
other gene names :
GPR31; GPR31; HETER; HETER1; 12-HETER; 12-(S)-HETE receptor; 12-HETER
uniprot entry name :
GPR31_HUMAN
reactivity :
Mouse
sequence length :
319
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant 12-HETE concentration. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: Up to 0.5 ng/ml. Assay Type: Sandwich
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse 12-HETE monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi gi num :
153792268
ncbi acc num :
NP_005290.2
ncbi gb acc num :
NM_005299.2
ncbi mol weight :
35,075 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Free Fatty Acid Receptors Pathway (161023); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)
uniprot summary :
GPR31: High-affinity receptor for 12-(S)-hydroxy-5,8,10,14- eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B. Belongs to the G-protein coupled receptor 1 family. Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR. Chromosomal Location of Human Ortholog: 6q27. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway
size1 :
48-Strip-Wells
price1 :
290 USD
size2 :
96-Strip-Wells
price2 :
450
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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