ELISA/assay

Human Caveolin 1 (CAV1) ELISA Kit

MBS265760

48-Strip-Wells

275 USD

ELISA Kit

Human Caveolin 1 (CAV1) ELISA Kit

Caveolin 1 (CAV1)

caveolin 1, partial; Caveolin-1; caveolin-1; cell growth-inhibiting protein 32; caveolin 1, caveolae protein, 22kDa

CAV1

CAV1; CAV1; CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085; CAV

CAV1_HUMAN

Human

113

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant CAV1 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human CAV1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4972627

AAD34722.1

17,023 Da

ALK1 Signaling Events Pathway (137968); Androgen Receptor Signaling Pathway (198806); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Basigin Interactions Pathway (106065); Canonical Wnt Signaling Pathway (138032); Cell Surface Interactions At The Vascular Wall Pathway (106062); Direct P53 Effectors Pathway (137939); EGFR1 Signaling Pathway (198782); Endocytosis Pathway (102279)

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]

Caveolin-1: May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)- mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3- dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin. Interacts with rotavirus A NSP4. Interacts (via the N- terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with BMX and BTK. Expressed in muscle and lung, less so in liver, brain and kidney. Belongs to the caveolin family. 2 isoforms of the human protein are produced by alternative initiation. Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 7q31.1. Cellular Component: focal adhesion; protein complex; endoplasmic reticulum; basolateral plasma membrane; integral to plasma membrane; lipid particle; cell cortex; caveola; acrosomal membrane; cilium; lipid raft; Golgi membrane; perinuclear region of cytoplasm; early endosome membrane; apical plasma membrane; plasma membrane; cytoplasmic vesicle; intracellular; endosome. Molecular Function: identical protein binding; protein binding; protease activator activity; enzyme binding; cholesterol binding; patched binding; protein complex scaffold; structural molecule activity; nitric-oxide synthase binding; protein kinase binding; receptor binding. Biological Process: mammary gland involution; viral reproduction; negative regulation of epithelial cell differentiation; negative regulation of tyrosine phosphorylation of Stat5 protein; nitric oxide homeostasis; negative regulation of BMP signaling pathway; calcium ion homeostasis; sequestering of lipid; protein localization; regulation of the force of heart contraction by chemical signal; regulation of fatty acid metabolic process; negative regulation of protein binding; inactivation of MAPK activity; regulation of smooth muscle contraction; maintenance of cellular protein localization; skeletal muscle development; cytosolic calcium ion homeostasis; negative regulation of nitric-oxide synthase activity; cellular response to starvation; membrane depolarization; cholesterol homeostasis; response to estrogen stimulus; negative regulation of endothelial cell proliferation; T cell costimulation; negative regulation of protein ubiquitination; regulation of nitric-oxide synthase activity; response to progesterone stimulus; response to calcium ion; leukocyte migration; negative regulation of JAK-STAT cascade; lactation; vesicle organization and biogenesis; negative regulation of peptidyl-serine phosphorylation; negative regulation of pinocytosis; negative regulation of transcription from RNA polymerase II promoter; nitric oxide metabolic process; mammary gland development; calcium ion transport; negative regulation of cytokine and chemokine mediated signaling pathway; angiogenesis; vasculogenesis; protein homooligomerization; vasoconstriction; cholesterol transport; negative regulation of MAPKKK cascade; negative regulation of nitric oxide biosynthetic process; MAPKKK cascade; positive regulation of metalloenzyme activity; positive regulation of peptidyl-serine phosphorylation; regulation of peptidase activity; cellular calcium ion homeostasis; triacylglycerol metabolic process; regulation of blood coagulation; positive regulation of vasoconstriction; response to hypoxia; vascular endothelial growth factor receptor signaling pathway; blood coagulation. Disease: Lipodystrophy, Congenital Generalized, Type 3; Partial Lipodystrophy, Congenital Cataracts, And Neurodegeneration Syndrome; Pulmonary Hypertension, Primary, 3

48-Strip-Wells

275 USD

96-Strip-Wells

420