Human Epidermal Growth Factor (EGF) ELISA Kit | MyBioSource MBS265750 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Epidermal Growth Factor (EGF) ELISA Kit

catalog :

MBS265750

quantity :

48-Strip-Wells

price :

250 USD

product information

catalog number :

MBS265750

products type :

ELISA Kit

products full name :

Human Epidermal Growth Factor (EGF) ELISA Kit

products short name :

[Epidermal Growth Factor (EGF)]

other names :

[epidermal growth factor, partial; Pro-epidermal growth factor; pro-epidermal growth factor; beta-urogastrone; epidermal growth factor; Urogastrone]

products gene name :

[EGF]

other gene names :

[EGF; EGF; URG; HOMG4; EGF]

uniprot entry name :

EGF_HUMAN

reactivity :

Human

sequence length :

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant EGF concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.

other info2 :

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human EGF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

46242544

ncbi acc num :

AAS83395.1

ncbi mol weight :

129,746 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Arf6 Signaling Events Pathway (138034); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Ceramide Signaling Pathway (138023); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550)

ncbi summary :

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

uniprot summary :

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ligand, receptor tyrosine kinase; Membrane protein, integral; Hormone. Chromosomal Location of Human Ortholog: 4q25. Cellular Component: extracellular space; lysosomal membrane; integral to membrane; extracellular region; plasma membrane. Molecular Function: transmembrane receptor protein tyrosine kinase activator activity; protein binding; growth factor activity; calcium ion binding; epidermal growth factor receptor binding. Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; transmembrane receptor protein tyrosine kinase activation (dimerization); peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; signal transduction; positive regulation of MAP kinase activity; platelet degranulation; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; angiogenesis; DNA replication; STAT protein nuclear translocation; positive regulation of granule cell precursor proliferation; positive regulation of DNA binding; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of mitosis; branching morphogenesis of a tube; innate immune response; negative regulation of secretion; blood coagulation; positive regulation of phosphorylation. Disease: Hypomagnesemia 4, Renal

size1 :

48-Strip-Wells

price1 :

250 USD

size2 :

96-Strip-Wells

price2 :

365

size3 :

5x96-Strip-Wells

price3 :

1480

size4 :

10x96-Strip-Wells

price4 :

2620