catalog number :
MBS265649
products type :
ELISA Kit
products full name :
Rat Kidney Injury Molecule 1 (Kim1) ELISA Kit
products short name :
[Kidney Injury Molecule 1 (Kim1)]
other names :
[kidney injury molecule 1; Kidney injury molecule 1; Kidney injury molecule 1; Uncharacterized protein; Zgc:92647]
products gene name :
[Kim1]
other gene names :
[havcr1; kim1; zgc:92647]
uniprot entry name :
Q6DH50_DANRE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve
other info1 :
Samples: Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant Kim-1 concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat Kim-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
ABP97434.1
ncbi mol weight :
29,575 Da
size4 :
10x96-Strip-Wells