ELISA/assay

Human alpha-Lactalbumin (alpha-La) ELISA Kit

MBS265606

48-Strip-Wells

275 USD

ELISA Kit

Human alpha-Lactalbumin (alpha-La) ELISA Kit

alpha-Lactalbumin (alpha-La)

Human a-Lactalbumin (a-La)  ELISA Kit

alpha-lactalbumin; Alpha-lactalbumin; alpha-lactalbumin; lysozyme-like protein 7; lactose synthase B protein; lactalbumin, alpha-; Lactose synthase B protein; Lysozyme-like protein 7

alpha-La

LALBA; LALBA; LYZL7

LALBA_HUMAN

Human

142

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant alpha-La concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human alpha-La monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4504947

NP_002280.1

NM_002289.2

16,225 Da

Galactose Metabolism Pathway (82931); Galactose Metabolism Pathway (292)

This gene encodes alpha-lactalbumin, a principal protein of milk. Alpha-lactalbumin forms the regulatory subunit of the lactose synthase (LS) heterodimer and beta 1,4-galactosyltransferase (beta4Gal-T1) forms the catalytic component. Together, these proteins enable LS to produce lactose by transfering galactose moieties to glucose. As a monomer, alpha-lactalbumin strongly binds calcium and zinc ions and may possess bactericidal or antitumor activity. A folding variant of alpha-lactalbumin, called HAMLET, likely induces apoptosis in tumor and immature cells. [provided by RefSeq, Jul 2008]

LALBA: Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins. Belongs to the glycosyl hydrolase 22 family. Protein type: Secreted, signal peptide; Secreted; Transferase; Apoptosis. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: extracellular space. Molecular Function: lactose synthase activity; calcium ion binding. Biological Process: cell-cell signaling; apoptosis; defense response to bacterium; signal transduction; lactose biosynthetic process

48-Strip-Wells

275 USD

96-Strip-Wells

420