catalog number :
MBS265422
products type :
ELISA Kit
products full name :
Mouse Alpha-1-Microglobulin (alpha1-MG) ELISA Kit
products short name :
Alpha-1-Microglobulin (alpha1-MG)
products name syn :
Mouse Alpha-1-Microglobulin (a1-MG) ELISA Kit
other names :
alpha-1-microglobulin, partial; Protein AMBP; protein AMBP; bikunin; uristatin; protein HC; trypstatin; uronic-acid-rich protein; growth-inhibiting protein 19; inter-alpha-trypsin inhibitor light chain; complex-forming glycoprotein heterogeneous in charge; alpha-1-microglobulin/bikunin precursor; Alpha-1 microglycoprotein; Complex-forming glycoprotein heterogeneous in charge
products gene name :
alpha1-MG
other gene names :
AMBP; AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC; HCP; ITIL; Protein HC; ITI-LC
uniprot entry name :
AMBP_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant alpha1-MG concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse alpha1-MG monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
CAA38587.1
ncbi mol weight :
38,999 Da
ncbi pathways :
Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Scavenging Of Heme From Plasma Pathway (771600)
ncbi summary :
This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]
uniprot summary :
AMBP: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9q32-q33. Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; extracellular region; plasma membrane. Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; IgA binding; heme binding; calcium oxalate binding; calcium channel inhibitor activity. Biological Process: receptor-mediated endocytosis; negative regulation of JNK cascade; viral reproduction; heme catabolic process; negative regulation of immune response; protein catabolic process; female pregnancy; cell adhesion; protein-chromophore linkage