Guinea pig Endothelin 1 (ET-1) ELISA Kit | MyBioSource MBS265321 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Guinea pig Endothelin 1 (ET-1) ELISA Kit

catalog :

MBS265321

quantity :

48-Strip-Wells

price :

275 USD

product information

catalog number :

MBS265321

products type :

ELISA Kit

products full name :

Guinea pig Endothelin 1 (ET-1) ELISA Kit

products short name :

[Endothelin 1 (ET-1)]

other names :

[endothelin-1; Endothelin-1; endothelin-1; preproendothelin-1; endothelin 1; Preproendothelin-1; PPET1]

products gene name :

[ET-1]

other gene names :

[EDN1; EDN1; ET1; QME; PPET1; ARCND3; HDLCQ7; PPET1; ET-1]

uniprot entry name :

EDN1_HUMAN

reactivity :

Guinea Pig

sequence length :

212

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Guinea pig serum, plasma or cell culture supernatant and organizations in the natural and recombinant ET-1 concentration. Assay Type: Sandwich. Detection Range: 500 pg/ml-7.8 pg/ml. Sensitivity: The minimum detectable Guinea pig ET-1 up to 1 pg/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Guinea pig ET-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

556203

ncbi acc num :

AAA52339.1

ncbi mol weight :

24,425 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); EGFR-dependent Endothelin Signaling Events Pathway (138037); Endothelin Pathway (672453); Endothelins Pathway (137958); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045)

ncbi summary :

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

uniprot summary :

EDN1: Endothelins are endothelium-derived vasoconstrictor peptides. Belongs to the endothelin/sarafotoxin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 6p24.1. Cellular Component: extracellular space; cytoplasm; extracellular region. Molecular Function: protein binding; hormone activity; endothelin B receptor binding; cytokine activity; endothelin A receptor binding. Biological Process: response to nicotine; positive regulation of JNK activity; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of nitric oxide biosynthetic process; regulation of systemic arterial blood pressure by endothelin; heart development; response to lipopolysaccharide; middle ear morphogenesis; prostaglandin biosynthetic process; sensory perception of pain; positive regulation of MAP kinase activity; elevation of cytosolic calcium ion concentration; negative regulation of cAMP biosynthetic process; cell surface receptor linked signal transduction; cell-cell signaling; negative regulation of nitric-oxide synthase biosynthetic process; protein kinase C activation; cell growth; neutrophil chemotaxis; rhythmic excitation; positive regulation of mitosis; negative regulation of blood coagulation; positive regulation of heart rate; respiratory gaseous exchange; response to testosterone stimulus; response to amino acid stimulus; peptide hormone secretion; leukocyte activation; patterning of blood vessels; membrane depolarization; protein kinase C deactivation; regulation of vasoconstriction; positive regulation of transcription from RNA polymerase II promoter; response to activity; superoxide release; positive regulation of odontogenesis; epithelial fluid transport; phosphoinositide 3-kinase cascade; G-protein signaling, phospholipase D activating pathway; positive regulation of cell size; neural crest cell development; negative regulation of hormone secretion; positive regulation of smooth muscle cell proliferation; negative regulation of cellular protein metabolic process; negative regulation of transcription from RNA polymerase II promoter; glucose transport; vein smooth muscle contraction; histamine secretion; nitric oxide transport; positive regulation of cell proliferation; regulation of pH; positive regulation of smooth muscle contraction; artery smooth muscle contraction; vasoconstriction; inositol phosphate-mediated signaling; in utero embryonic development; positive regulation of hormone secretion; calcium-mediated signaling; multicellular organismal aging; body fluid secretion; G-protein coupled receptor protein signaling pathway; dorsal/ventral pattern formation; cartilage development; response to ozone; positive regulation of prostaglandin secretion; maternal process involved in parturition; regulation of sensory perception of pain; positive regulation of cell migration. Disease: Question Mark Ears, Isolated; Auriculocondylar Syndrome 3

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

435

size3 :

5x96-Strip-Wells

price3 :

1755

size4 :

10x96-Strip-Wells

price4 :

3150