ELISA/assay

Rat Transferrin Receptor (TFR) ELISA Kit

MBS265236

48-Strip-Wells

290 USD

ELISA Kit

Rat Transferrin Receptor (TFR) ELISA Kit

Transferrin Receptor (TFR)

transferrin receptor; Transferrin receptor protein 1; transferrin receptor protein 1; transferrin receptor (p90, CD71); transferrin receptor; T9; p90; CD_antigen: CD71Cleaved into the following chain:Transferrin receptor protein 1, serum form; sTfR

TFR

TFRC; TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR; TR; TfR; TfR1; Trfr; sTfR

TFR1_HUMAN

Rat

760

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TFR concentration. Assay Type: Sandwich. Detection Range: 50 ng/ml-0.78 ng/ml. Sensitivity: 0.08 ng/ml.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat TFR monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

339516

AAA61153.1

84,871 Da

Clathrin Derived Vesicle Budding Pathway (119545); Endocytosis Pathway (102279); Endocytosis Pathway (102181); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Golgi Associated Vesicle Biogenesis Pathway (119546); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Iron Metabolism In Placenta Pathway (672461)

TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Protein type: Cell surface; Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: 3q29. Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasmic membrane-bound vesicle; extracellular region; coated pit; recycling endosome; membrane; perinuclear region of cytoplasm; plasma membrane; melanosome; vesicle; endosome; external side of plasma membrane. Molecular Function: identical protein binding; protein binding; transferrin receptor activity; double-stranded RNA binding. Biological Process: receptor-mediated endocytosis; viral reproduction; cellular iron ion homeostasis; positive regulation of bone resorption; transferrin transport; osteoclast differentiation; regulation of cell growth; transmembrane transport; regulation of cell proliferation

48-Strip-Wells

290 USD

96-Strip-Wells

450