ELISA/assay

Mouse alpha-fetoprotein (AFP) ELISA Kit

MBS265233

48-Strip-Wells

290 USD

ELISA Kit

Mouse alpha-fetoprotein (AFP) ELISA Kit

alpha-fetoprotein (AFP)

Mouse a-fetoprotein (AFP) ELISA Kit

alpha-fetoprotein; Alpha-fetoprotein; alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein; alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

AFP

AFP; AFP; AFPD; FETA; HPAFP; HPAFP

FETA_HUMAN

Mouse

609

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant AFP concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse AFP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4501989

NP_001125.1

NM_001134.2

68,678 Da

Direct P53 Effectors Pathway (137939); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Glucocorticoid Receptor Regulatory Network Pathway (138014); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388)

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular space; cytoplasm. Molecular Function: metal ion binding. Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle. Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of

48-Strip-Wells

290 USD

96-Strip-Wells

450