ELISA/assay

Guinea pig Protein C ELISA Kit

MBS265222

48-Strip-Wells

315 USD

ELISA Kit

Guinea pig Protein C ELISA Kit

Protein C

protein C, partial; Vitamin K-dependent protein C; vitamin K-dependent protein C; prepro-protein C; autoprothrombin IIA; anticoagulant protein C; blood coagulation factor XIV; protein C (inactivator of coagulation factors Va and VIIIa); Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIVCleaved into the following 3 chains:Vitamin K-dependent protein C light chain; Vitamin K-dependent protein C heavy chain; Activation peptide

PROC; PROC; PC; APC; PROC1; THPH3; THPH4

PROC_HUMAN

Guinea Pig

84

Store all reagents at 2-8 degree C.

Samples: Guinea pig serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PC concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Guinea pig PC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

265668

AAB25410.1

57,556 Da

Cell Surface Interactions At The Vascular Wall Pathway (106062); Common Pathway (106060); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Gamma-carboxylation Of Protein Precursors Pathway (106233); Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway (106232); Hemostasis Pathway (106028); Metabolism Of Proteins Pathway (106230)

This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.[provided by RefSeq, Dec 2009]

PROC: Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Defects in PROC are the cause of thrombophilia due to protein C deficiency, autosomal dominant (THPH3). A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Individuals with decreased amounts of protein C are classically referred to as having type I protein C deficiency and those with normal amounts of a functionally defective protein as having type II deficiency. Defects in PROC are the cause of thrombophilia due to protein C deficiency, autosomal recessive (THPH4). A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. It results in a thrombotic condition that can manifest as a severe neonatal disorder or as a milder disorder with late-onset thrombophilia. The severe form leads to neonatal death through massive neonatal venous thrombosis. Often associated with ecchymotic skin lesions which can turn necrotic called purpura fulminans, this disorder is very rare. Belongs to the peptidase S1 family. Protein type: EC 3.4.21.69; Apoptosis; Protease. Chromosomal Location of Human Ortholog: 2q13-q14. Cellular Component: endoplasmic reticulum lumen; Golgi lumen; extracellular region. Molecular Function: protein binding; serine-type endopeptidase activity; calcium ion binding. Biological Process: cellular protein metabolic process; negative regulation of blood coagulation; post-translational protein modification; proteolysis; blood coagulation; leukocyte migration; peptidyl-glutamic acid carboxylation; negative regulation of apoptosis. Disease: Thrombophilia Due To Protein C Deficiency, Autosomal Recessive; Thrombophilia Due To Protein C Deficiency, Autosomal Dominant

48-Strip-Wells

315 USD

96-Strip-Wells

490