ELISA/assay

Mouse Low Density Lipoprotein Receptor Related Protein 6 (LRP6) ELISA Kit

MBS265158

48-Strip-Wells

290 USD

ELISA Kit

Mouse Low Density Lipoprotein Receptor Related Protein 6 (LRP6) ELISA Kit

Low Density Lipoprotein Receptor Related Protein 6 (LRP6)

low-density lipoprotein receptor-related protein 6; Low-density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP-6; low density lipoprotein receptor-related protein 6

LRP6

LRP6; LRP6; ADCAD2; LRP-6

LRP6_HUMAN

Mouse

1613

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant LRP6 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse LRP6 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

148727288

NP_002327.2

NM_002336.2

180,429 Da

Canonical Wnt Signaling Pathway (138032); MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784); Presenilin Action In Notch And Wnt Signaling Pathway (137914); Wnt Signaling Pathway NetPath (198799); Wnt Signaling Pathway And Pluripotency (198847); Wnt Signaling Network Pathway (137962); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471)

This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.[provided by RefSeq, Dec 2009]

LRP6: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation. Homodimer; disulfide-linked. Forms phosphorylated oligomer aggregates on Wnt-signaling. Forms a WNT-signaling complex formed of a WNT protein, a FZD protein and LRP5 or LRP6. Interacts (via the extracellular domain) with WNT1; the interaction is enhanced by prior formation of the Wnt/Fzd complex. Interacts (via the beta-propeller regions 3 and 4) with WNT3A. Interacts (via the beta-propeller regions 1 and 2) with WNT9B. Interacts with FZD5; the interaction forms a coreceptor complex for Wnt signaling and is inhibited by DKK1 and C1orf187. Interacts (via beta propeller region) with DKK1; the interaction inhibits FZD5/LRP6 complex formation. Interacts with DKK2. Interacts with C1orf187/DRAXIN; the interaction inhibits Wnt signaling. Interacts (via the phosphorylated PPPSP motifs) with AXIN1; the interaction recruits the AXIN1/GSK3B complex to cell surface LRP6 signalsomes. Interacts with GRB10; the interaction prevents AXIN1 binding, thus negatively regulating the Wnt signaling pathway. Interacts (via the extracellular domain) with RSPO1; the interaction activates Wnt/beta-catenin signaling. Interacts (via the extracellular domain) with RSPO3 (via the cysteine rich domain); the interaction activates Wnt/beta-catenin signaling. Interacts (via the beta- propeller regions 1 and 2) with SOST; the interaction competes with DKK1 for binding for inhibiting beta-catenin signaling. Interacts with MESD; the interaction prevents the formation of LRP6 aggregates and targets LRP6 to the plasma membrane. Interacts (via the cytoplasmic domain) with CSNKIE; the interaction phosphorylates LRP6, binds AXIN1 and inhibits AXIN1/GSK3B-mediated phosphorylation of beta-catenin. Interacts with MACF1. Decreased levels on WNT3A stimulation. Widely co-expressed with LRP5 during embryogenesis and in adult tissues. Belongs to the LDLR family. Protein type: Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13.2. Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; early endosome; extracellular region; integral to membrane; caveola; cell soma; early endosome membrane; plasma membrane; synapse; cytoplasmic vesicle; receptor complex. Molecular Function: low-density lipoprotein receptor activity; Wnt-protein binding; identical protein binding; protein binding; Wnt receptor activity; protein homodimerization activity; kinase inhibitor activity; frizzled binding; toxin transporter activity; apolipoprotein binding; receptor binding. Biological Process: embryonic forelimb morphogenesis; response to peptide hormone stimulus; radial glial cell differentiation in the forebrain; positive regulation of apoptosis; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; cerebellum morphogenesis; Wnt receptor signaling pathway through beta-catenin; palate development; thalamus development; embryonic pattern specification; bone remodeling; embryonic hindlimb morphogenesis; Wnt receptor signaling pathway in forebrain neuroblast division; anterior/posterior pattern formation; synaptic transmission; elevation of cytosolic calcium ion concentration; cell-cell adhesion; negative regulation of protein amino acid phosphorylation; convergent extension; response to folic acid; positive regulation of mesenchymal cell proliferation; midbrain development; positive regulation of bone resorption; neural crest cell differentiation; heart looping; cell migration involved in gastrulation; embryonic limb morphogenesis; negative regulation of epithelial cell proliferation; Wnt receptor signaling pathway; external genitalia morphogenesis; neural crest formation; positive regulation of cell cycle; gastrulation with mouth forming second; negative regulation of fat cell differentiation; positive regulation of ossification; odontogenesis of dentine-containing teeth; formation of radial glial scaffolds; cerebral cortex cell migration; embryonic retina morphogenesis in camera-type eye; neural tube closure; midbrain-hindbrain boundary development; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; cerebral cortex development; embryonic camera-type eye morphogenesis; embryonic digit morphogenesis; regulation of ossification. Disease: Coronary Artery Disease, Autosomal Dominant 2

48-Strip-Wells

290 USD

96-Strip-Wells

450