ELISA/assay

Porcine Noradrenaline (NE) ELISA KIT

MBS265099

48-Strip-Wells

315 USD

ELISA Kit

Porcine Noradrenaline (NE) ELISA KIT

Noradrenaline (NE)

noradrenaline transporter; Sodium-dependent noradrenaline transporter; sodium-dependent noradrenaline transporter; norepinephrine transporter; neurotransmitter transporter; solute carrier family 6 member 5; solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2; solute carrier family 6 (neurotransmitter transporter), member 2; Norepinephrine transporter; NET; Solute carrier family 6 member 2

NE

SLC6A2; SLC6A2; NET; NAT1; NET1; SLC6A5; NAT1; NET1; SLC6A5; NET

SC6A2_HUMAN

Porcine

617

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Porcine serum, plasma or cell culture supernatant and organizations in the natural and recombinant NE concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine NE monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

189258

AAA59943.1

57,370 Da

Monoamine Transport Pathway (198851); Na+/Cl- Dependent Neurotransmitter Transporters Pathway (161070); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (119571)

This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

SLC6A2: Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A2 are a cause of orthostatic intolerance (OI). OI is a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. It is associated with postural tachycardia. Plasma norepinephrine concentration is abnormally high. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily. Protein type: Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral; Transporter. Chromosomal Location of Human Ortholog: 16q12.2. Cellular Component: neuron projection; cell surface; membrane; integral to plasma membrane; plasma membrane; lipid raft. Molecular Function: protein binding; monoamine transmembrane transporter activity; beta-tubulin binding; alpha-tubulin binding; norephinephrine:sodium symporter activity. Biological Process: response to drug; synaptic transmission; norepinephrine transport; transport; neurotransmitter transport; monoamine transport; response to pain; transmembrane transport. Disease: Orthostatic Intolerance

48-Strip-Wells

315 USD

96-Strip-Wells

490