catalog number :
MBS265079
products type :
ELISA Kit
products full name :
Mouse soluble cluster of differentiation 14 (sCD14) ELISA Kit
products short name :
soluble cluster of differentiation 14 (sCD14)
other names :
Sodium/hydrogen exchanger 10; Sodium/hydrogen exchanger 10; sodium/hydrogen exchanger 10; sNHE; NHE-10; Na(+)/H(+) exchanger 10; solute carrier family 9 member C1; solute carrier family 10 member 10; solute carrier family 9, member 10; solute carrier family 9, isoform 10; sperm-specific Na(+)/H(+) exchanger; sperm-specific sodium proton exchanger; solute carrier family 9, subfamily C (Na+-transporting carboxylic acid decarboxylase), member 1; Na(+)/H(+) exchanger 10; NHE-10; Solute carrier family 9 member 10; Solute carrier family 9 member C1; Sperm-specific Na(+)/H(+) exchanger; sNHE
products gene name :
sCD14
other gene names :
SLC9C1; SLC9C1; NHE; SLC9A10; sperm-NHE; SLC9A10; NHE-10; sNHE
uniprot entry name :
SL9C1_HUMAN
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant sCD14 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse sCD14 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi mol weight :
129,365 Da
ncbi pathways :
Ion Channel Transport Pathway (187193); Stimuli-sensing Channels Pathway (771568); Transmembrane Transport Of Small Molecules Pathway (106572)
ncbi summary :
SLC9A10 is a member of the sodium-hydrogen exchanger (NHE) family (see SLC9A1, MIM 107310) and is required for male fertility and sperm motility (Wang et al., 2003 [PubMed 14634667]).[supplied by OMIM, Apr 2009]
uniprot summary :
NHE10: Sperm-specific sodium/hydrogen exchanger involved in intracellular pH regulation of spermatozoa. Required for sperm motility and fertility. Involved in sperm cell hyperactivation, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. Required for the expression and bicarbonate regulation of the soluble adenylyl cyclase (sAC). Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, ion channel. Chromosomal Location of Human Ortholog: 3q13.2. Cellular Component: plasma membrane; integral to membrane. Molecular Function: solute:hydrogen antiporter activity. Biological Process: sperm motility; multicellular organismal development; sodium ion transport; spermatogenesis; cell differentiation; transmembrane transport