catalog number :
MBS265046
products type :
ELISA Kit
products full name :
Human secreted phospholipase A2 (sPLA2) ELISA Kit
products short name :
secreted phospholipase A2 (sPLA2)
other names :
secretory phospholipase A2, isoform C; CG11124 gene product from transcript CG11124-RC; CG11124-PA; CG11124-PB; CG11124-PC; sPLA2-PA; sPLA2-PB; sPLA2-PC; secreted phospholipase A2; secretory phospholipase A2; secretory Phospholipase A2
products gene name :
sPLA2
other gene names :
sPLA2; CG11124; CG14507; DmelCG11124; secretory phospholipase A[[2]]; sPLA[[2]]
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant sPLA2 concentration. Assay Type: Sandwich. Detection Range: 200 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human sPLA2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_001014501.1
ncbi gb acc num :
NM_001014501.3
ncbi pathways :
Arachidonic Acid Metabolism Pathway (3028); Arachidonic Acid Metabolism Pathway (366); Ether Lipid Metabolism Pathway (3027); Ether Lipid Metabolism Pathway (365); Glycerophospholipid Metabolism Pathway (3026); Glycerophospholipid Metabolism Pathway (364); Linoleic Acid Metabolism Pathway (3029); Linoleic Acid Metabolism Pathway (367); Alpha-Linolenic Acid Metabolism Pathway (3030); Alpha-Linolenic Acid Metabolism Pathway (368)