ELISA/assay

Human Macrophage Colony-Stimulating Factor Receptor (M-CSFR) ELISA kit

MBS264973

48-Strip-Wells

275 USD

ELISA Kit

Human Macrophage Colony-Stimulating Factor Receptor (M-CSFR) ELISA kit

Macrophage Colony-Stimulating Factor Receptor (M-CSFR)

macrophage colony-stimulating factor 1 receptor; Macrophage colony-stimulating factor 1 receptor; macrophage colony-stimulating factor 1 receptor; CD115 antigen; CSF-1 receptor; FMS proto-oncogene; proto-oncogene c-Fms; macrophage colony stimulating factor I receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; colony stimulating factor 1 receptor; CSF-1 receptor (EC:2.7.10.1); CSF-1-R; CSF-1R; M-CSF-R; Proto-oncogene c-Fms; CD_antigen: CD115

M-CSFR

CSF1R; CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R; FMS; CSF-1-R; CSF-1R; M-CSF-R

CSF1R_HUMAN

Human

972

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant M-CSFR concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human M-CSFR monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

27262659

NP_005202.2

NM_005211.3

33,248 Da

C-MYB Transcription Factor Network Pathway (138073); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Integrins In Angiogenesis Pathway (137999); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096)

The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

CSFR: an oncogenic tyrosine kinase receptor for CSF-1 (M-CSF). Drives growth and development of monocytes. Binding of CSF-1 induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins. There are at least five major tyrosine autophosphorylation sites. Two point mutations seen in 10-20% of patients with acute myeloid leukemia, chronic myelomonocytic leukemia or myelodysplasia. One mutation appears to be both somatic and germline, and disrupts Cbl binding and receptor turnover. v-fms lacks the Cbl binding site and causes feline leukemia. Mutations may also develop after chemotherapy for lymphoma. A distinct point mutation was found in some cases of hepatocellular carcinoma and related to increased expression, and another mutation was found in 2 of 40 patients with idiopathic myelofibrosis. Expression is elevated in breast tumors and cell lines, and expression in xenografts and transgenic mice has been correlated with xenograft growth and breast cancer development. Inhibitors: Ki-20227 and other Kit/PDGFR inhibitors. Protein type: Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; Kinase, protein; Oncoprotein; TK group; PDGFR family. Chromosomal Location of Human Ortholog: 5q32. Cellular Component: cell surface; integral to plasma membrane; plasma membrane; receptor complex. Molecular Function: macrophage colony stimulating factor receptor activity; protein homodimerization activity; cytokine binding; protein phosphatase binding; ATP binding. Biological Process: regulation of bone resorption; phosphoinositide-mediated signaling; peptidyl-tyrosine phosphorylation; macrophage differentiation; monocyte differentiation; multicellular organismal development; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; osteoclast differentiation; signal transduction; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of cell shape; cell proliferation; positive regulation of cell proliferation; ruffle organization and biogenesis; innate immune response; hemopoiesis; phosphatidylinositol metabolic process; positive regulation of protein amino acid phosphorylation; intercellular junction maintenance; inflammatory response; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration. Disease: Leukoencephalopathy, Diffuse Hereditary, With Spheroids

48-Strip-Wells

275 USD

96-Strip-Wells

420