ELISA/assay

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

MBS264965

48-Strip-Wells

275 USD

ELISA Kit

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

A Disintegrin And Metalloprotease 10 (ADAM10)

A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform 1 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 10; A disintegrin and metalloproteinase with thrombospondin motifs 10; ADAM-TS 10; zinc metalloendopeptidase; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 10; ADAM metallopeptidase with thrombospondin type 1 motif, 10

ADAM10

ADAMTS10; ADAMTS10; WMS; WMS1; ADAM-TS10; ADAMTS-10; ADAM-TS 10; ADAM-TS10; ADAMTS-10

ATS10_HUMAN

Human

1103

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ADAM10 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ADAM10 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

537544566

NP_112219.3

NM_030957.3

62,530 Da

Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (980460); O-linked Glycosylation Pathway (980459); Post-translational Protein Modification Pathway (161012)

This gene belongs to the ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 motifs) family of zinc-dependent proteases. ADAMTS proteases are complex secreted enzymes containing a prometalloprotease domain of the reprolysin type attached to an ancillary domain with a highly conserved structure that includes at least one thrombospondin type 1 repeat. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. The product of this gene plays a major role in growth and in skin, lens, and heart development. It is also a candidate gene for autosomal recessive Weill-Marchesani syndrome. [provided by RefSeq, Jul 2008]

ADAMTS10: Metalloprotease that participate in microfibrils assembly. Microfibrils are extracellular matrix components occurring independently or along with elastin in the formation of elastic tissues. Defects in ADAMTS10 are the cause of Weill-Marchesani syndrome 1 (WMS1). WMS1 is a rare connective tissue disorder characterized by short stature, brachydactyly, joint stiffness, and eye abnormalities including microspherophakia, ectopia lentis, severe myopia and glaucoma. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Protease; EC 3.4.24.-; Secreted. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: extracellular matrix; microfibril. Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity. Biological Process: proteolysis. Disease: Weill-marchesani Syndrome 1

48-Strip-Wells

275 USD

96-Strip-Wells

420