ELISA/assay

Rat Pancreatic Polypeptide (PP) ELISA Kit

MBS264952

48-Strip-Wells

290 USD

ELISA Kit

Rat Pancreatic Polypeptide (PP) ELISA Kit

Pancreatic Polypeptide (PP)

pancreatic polypeptide; Pancreatic prohormone; pancreatic prohormone; PP; pancreatic polypeptide Y; prepro-PP (prepropancreatic polypeptide); pancreatic polypeptide; Pancreatic polypeptide; PP; INN: ObinepitideCleaved into the following 2 chains:Pancreatic hormone; PH; Pancreatic icosapeptide; PI

PP

PPY; PPY; PNP; PNP; PP; PH; PI

PAHO_HUMAN

Rat

95

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PP concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat PP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

119572034

EAW51649.1

10,445 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. [provided by RefSeq, Jul 2008]

PPY: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. Belongs to the NPY family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: extracellular space; extracellular region. Molecular Function: neuropeptide hormone activity; G-protein-coupled receptor binding; hormone activity; receptor binding. Biological Process: cell-cell signaling; neuropeptide signaling pathway; regulation of appetite; digestion; protein secretion; feeding behavior

48-Strip-Wells

290 USD

96-Strip-Wells

450