ELISA/assay

Human Corticotropin Releasing Hormone (CRH) ELISA Kit

MBS264947

48-Strip-Wells

275 USD

ELISA Kit

Human Corticotropin Releasing Hormone (CRH) ELISA Kit

Corticotropin Releasing Hormone (CRH)

corticotropin releasing hormone; Corticoliberin; corticoliberin; corticotropin-releasing factor; corticotropin releasing hormone; Corticotropin-releasing factor; CRF; Corticotropin-releasing hormone

CRH

CRH; CRH; CRF; CRF

CRF_HUMAN

Human

196

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant CRH concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human CRH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

119607303

EAW86897.1

21,422 Da

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Class B/2 (Secretin Family Receptors) Pathway (106378); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Long-term Depression Pathway (83086); Long-term Depression Pathway (497); Myometrial Relaxation And Contraction Pathways (198759); Rapid Glucocorticoid Signaling Pathway (137923)

Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Apr 2010]

CRH: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8q13. Cellular Component: extracellular space; cytoplasm; extracellular region; perikaryon. Molecular Function: corticotropin-releasing hormone receptor 1 binding; neuropeptide hormone activity; protein binding; hormone activity; corticotropin-releasing hormone receptor 2 binding; receptor binding; adrenocorticotropin-releasing hormone activity. Biological Process: synaptic transmission, dopaminergic; diterpenoid metabolic process; negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hypothalamus development; induction of apoptosis by hormones; response to pain; female pregnancy; signal transduction; positive regulation of adrenocorticotropic hormone secretion; synaptic transmission; negative regulation of luteinizing hormone secretion; learning and/or memory; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; feeding behavior; negative regulation of blood pressure; parturition; response to electrical stimulus; inflammatory response; associative learning; response to corticosterone stimulus; response to drug; adrenal gland development; response to ether; response to ethanol; long-term memory; ion homeostasis; response to estrogen stimulus; regulation of serotonin secretion; negative regulation of epinephrine secretion; positive regulation of protein amino acid phosphorylation; glucocorticoid biosynthetic process; lung development. Disease: Acth Deficiency, Isolated

48-Strip-Wells

275 USD

96-Strip-Wells

420