catalog number :
MBS264912
products type :
ELISA Kit
products full name :
Mouse Thrombin/Antithrombin Complex (TAT) ELISA KIT
products short name :
Thrombin/Antithrombin Complex (TAT)
other names :
Tat; p14; Tat
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TAT concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse TAT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_057853.1
ncbi gb acc num :
NC_001802.1
ncbi pathways :
Disease Pathway (530764); Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway (106004); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984); HIV Transcription Elongation Pathway (106001); Host Interactions Of HIV Factors Pathway (106010); Interactions Of Tat With Host Cellular Proteins Pathway (106018); Late Phase Of HIV Life Cycle Pathway (105995); Pausing And Recovery Of Tat-mediated HIV Elongation Pathway (161055); Signaling Events Mediated By HDAC Class III Pathway (137972)