ELISA/assay

Mouse Apolipoprotein B100 (Apo-B100) ELISA Kit

MBS264905

96 Strip Wells

450 USD

ELISA Kit

Mouse Apolipoprotein B100 (Apo-B100) ELISA Kit

Apolipoprotein B100 (Apo-B100)

apolipoprotein B-100; Apolipoprotein B-100; apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen); apolipoprotein B

Apo-B100

APOB; APOB; FLDB; LDLCQ4; Apo B-100; Apo B-48

APOB_HUMAN

Mouse

4563

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: This product is suitable for in vitro quantitative detection of Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant Apo-B100 concentration. Detection Range: 2000 ng/ml-31.2 ng/ml

Sensitivity: the minimum detectable Mouse Apo-B100up to 12 ng/ml. Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse Apo-B100 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

105990532

NP_000375.2

NM_000384.2

515,605 Da

Binding And Uptake Of Ligands By Scavenger Receptors Pathway 771599!!Cell Surface Interactions At The Vascular Wall Pathway 106062!!Chylomicron-mediated Lipid Transport Pathway 106157!!Disease Pathway 530764!!Diseases Associated With Visual Transduction Pathway 771581!!FOXA1 Transcription Factor Network Pathway 137979!!Fat Digestion And Absorption Pathway 194385!!Fat Digestion And Absorption Pathway 194324!!Hemostasis Pathway 106028!!LDL-mediated Lipid Transport Pathway 106159

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA- UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

96 Strip Wells

450 USD