catalog number :
MBS264847
products type :
ELISA Kit
products full name :
Human Asymmetric dimethylarginine (ADMA) ELISA Kit
products short name :
Asymmetric dimethylarginine (ADMA)
other names :
Alanine--glyoxylate aminotransferase 2, mitochondrial; Alanine--glyoxylate aminotransferase 2, mitochondrial; alanine--glyoxylate aminotransferase 2, mitochondrial; beta-ALAAT II; beta-alanine-pyruvate aminotransferase; (R)-3-amino-2-methylpropionate--pyruvate transaminase; alanine--glyoxylate aminotransferase 2; (R)-3-amino-2-methylpropionate--pyruvate transaminase (EC:2.6.1.40); Beta-ALAAT II; Beta-alanine-pyruvate aminotransferase; D-AIBAT
products gene name :
ADMA
other gene names :
AGXT2; AGXT2; AGT2; DAIBAT; AGT2; AGT 2
uniprot entry name :
AGT2_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant ADMA concentration. Assay Type: Quantitative Sandwich. Sensitivity: Up to 0.01umol/L.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ADMA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi mol weight :
49,297 Da
ncbi pathways :
Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Glycine, Serine And Threonine Metabolism Pathway (82949); Glycine, Serine And Threonine Metabolism Pathway (313); Glyoxylate Metabolism Pathway (160990); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Metabolism Of Nucleotides Pathway (106263); Pyrimidine Catabolism Pathway (106288)
ncbi summary :
The protein encoded by this gene is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. [provided by RefSeq, Dec 2008]
uniprot summary :
AGXT2: is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. [provided by RefSeq, Dec 2008]. Protein type: EC 2.6.1.40; Mitochondrial; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.44; Transferase. Chromosomal Location of Human Ortholog: 5p13. Cellular Component: mitochondrion; mitochondrial matrix. Molecular Function: beta-alanine-pyruvate transaminase activity; alanine-glyoxylate transaminase activity; (R)-3-amino-2-methylpropionate-pyruvate transaminase activity; pyridoxal phosphate binding. Biological Process: pyrimidine nucleoside catabolic process; positive regulation of nitric oxide biosynthetic process; glyoxylate catabolic process; pyrimidine base metabolic process; glyoxylate metabolic process; nucleobase, nucleoside and nucleotide metabolic process; glycine biosynthetic process, by transamination of glyoxylate; L-alanine catabolic process, by transamination