ELISA/assay

Rat Heparin Binding Epidermal Growth Factor Like Growth Factor (HB-EGF) ELISA Kit

MBS264729

48-Strip-Wells

290 USD

ELISA Kit

Rat Heparin Binding Epidermal Growth Factor Like Growth Factor (HB-EGF) ELISA Kit

Heparin Binding Epidermal Growth Factor Like Growth Factor (HB-EGF)

heparin-binding epidermal growth factor-like growth factor; Proheparin-binding EGF-like growth factor; proheparin-binding EGF-like growth factor; diphtheria toxin receptor; heparin binding epidermal growth factor-like growth factor; heparin-binding EGF-like growth factor

HB-EGF

Hbegf; Hbegf; Dtr; Dts; Hegfl; AW047313; Dtr; Hegfl; HB-EGF; HBEGF

HBEGF_MOUSE

Rat

208

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant HB-EGF concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat HB-EGF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

1236400

AAC52617.1

22,808 Da

Adaptive Immune System Pathway (819568); Constitutive PI3K/AKT Signaling In Cancer Pathway (819551); DAP12 Interactions Pathway (819675); DAP12 Signaling Pathway (819676); Disease Pathway (819462); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (819582); Downstream Signal Transduction Pathway (819799); Downstream Signaling Of Activated FGFR Pathway (819508); EGFR Transactivation By Gastrin Pathway (819889); ErbB Signaling Pathway (83246)

HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. Protein type: Membrane protein, integral; Cell surface. Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; integral to membrane; plasma membrane; extracellular region. Molecular Function: heparin binding; growth factor activity; epidermal growth factor receptor binding. Biological Process: positive regulation of keratinocyte migration; epidermal growth factor receptor signaling pathway; positive regulation of protein kinase B signaling cascade; cell migration; positive regulation of smooth muscle cell proliferation; negative regulation of elastin biosynthetic process; blastocyst growth; angiogenesis; positive regulation of cell growth; regulation of heart contraction; positive regulation of cell migration; wound healing, spreading of epidermal cells

48-Strip-Wells

290 USD

96-Strip-Wells

450