ELISA/assay

Mouse Alanine Aminotransferase (ALT) ELISA Kit

MBS264717

48-Strip-Wells

290 USD

ELISA Kit

Mouse Alanine Aminotransferase (ALT) ELISA Kit

Alanine Aminotransferase (ALT)

alanine aminotransferase, partial; Alanine aminotransferase 1; alanine aminotransferase 1; GPT 1; glutamic-alanine transaminase 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1; glutamic-pyruvate transaminase (alanine aminotransferase); Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1

ALT

GPT; GPT; AAT1; ALT1; GPT1; AAT1; GPT1; ALT1; GPT 1

ALAT1_HUMAN

Mouse

493

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ALT concentration. Assay Type: Sandwich. Detection Range: 100 U/L-1.56 U/L. Sensitivity: 0.5 U/L.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse ALT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

1507680

BAA01186.1

54,637 Da

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (106173); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169)

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]

GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. Protein type: EC 2.6.1.2; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: cytosol. Molecular Function: alanine transaminase activity; pyridoxal phosphate binding. Biological Process: amino acid biosynthetic process; L-alanine catabolic process

48-Strip-Wells

290 USD

96-Strip-Wells

450