catalog number :
MBS264677
products type :
ELISA Kit
products full name :
Mouse 50% complement hemolysis (CH50) ELISA Kit
products short name :
50% complement hemolysis (CH50)
other names :
complement C4; Complement C4; complement C4
products gene name :
CH50
other gene names :
C4A; C4; C4
uniprot entry name :
CO4_BOVIN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant CH50 concentration. Assay Type: Sandwich. Detection Range: 100 U/ml-1.56U/ml. Sensitivity: Up to 0.5 U/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse CH50 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_001159957.1
ncbi gb acc num :
NM_001166485.1
ncbi mol weight :
101,885 Da
ncbi pathways :
Complement Activation, Classical Pathway (920897); Complement And Coagulation Cascades Pathway (84312); Complement And Coagulation Cascades Pathway (484); Pertussis Pathway (218110); Pertussis Pathway (218099); Staphylococcus Aureus Infection Pathway (172831); Staphylococcus Aureus Infection Pathway (171867); Systemic Lupus Erythematosus Pathway (84357); Systemic Lupus Erythematosus Pathway (534)