Human Vitamin D3 (VD3) ELISA kit | MyBioSource MBS264661 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Vitamin D3 (VD3) ELISA kit

catalog :

MBS264661

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS264661

products type :

ELISA Kit

products full name :

Human Vitamin D3 (VD3) ELISA kit

products short name :

Vitamin D3 (VD3)

other names :

vitamin D3 receptor isoform VDRA; Vitamin D3 receptor; vitamin D3 receptor; 1,25-dihydroxyvitamin D3 receptor; vitamin D nuclear receptor variant 1; nuclear receptor subfamily 1 group I member 1; protein phosphatase 1, regulatory subunit 163; vitamin D (1,25- dihydroxyvitamin D3) receptor; 1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1

products gene name :

VD3

other gene names :

VDR; VDR; NR1I1; PPP1R163; NR1I1; VDR

uniprot entry name :

VDR_HUMAN

reactivity :

Human

sequence length :

427

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant VD3 concentration. Assay Type: Sandwich. Detection Range: 500 ng/ml-7.8 ng/ml. Sensitivity: 1 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

. Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human VD3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

63054845

ncbi acc num :

NP_001017535.1

ncbi gb acc num :

NM_001017535.1

ncbi mol weight :

53,883 Da

ncbi pathways :

Direct P53 Effectors Pathway (137939); Drug Induction Of Bile Acid Pathway (698755); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213307); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Nuclear Receptor Transcription Pathway (105979); Nuclear Receptors Pathway (198848)

ncbi summary :

This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Feb 2011]

uniprot summary :

VDR: Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. Defects in VDR are the cause of rickets vitamin D- dependent type 2A (VDDR2A). A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. Belongs to the nuclear hormone receptor family. NR1 subfamily. Protein type: Nuclear receptor; Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 12q13.11. Cellular Component: nucleoplasm; nucleus; receptor complex. Molecular Function: retinoid X receptor binding; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; vitamin D3 receptor activity; transcription factor activity. Biological Process: lactation; transcription initiation from RNA polymerase II promoter; positive regulation of apoptosis involved in mammary gland involution; cell morphogenesis; decidualization; negative regulation of transcription from RNA polymerase II promoter; signal transduction; intestinal absorption; cellular calcium ion homeostasis; negative regulation of cell proliferation; calcium ion transport; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; gene expression; negative regulation of transcription, DNA-dependent; skeletal development; positive regulation of keratinocyte differentiation. Disease: Vitamin D-dependent Rickets, Type 2a; Osteoporosis

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage