Human Thrombopoietin (TPO) ELISA Kit | MyBioSource MBS264604 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Thrombopoietin (TPO) ELISA Kit

catalog :

MBS264604

quantity :

48-Strip-Wells

price :

250 USD

product information

catalog number :

MBS264604

products type :

ELISA Kit

products full name :

Human Thrombopoietin (TPO) ELISA Kit

products short name :

[Thrombopoietin (TPO)]

other names :

[thrombopoietin; Thrombopoietin; thrombopoietin; MPL ligand; c-mpl ligand; prepro-thrombopoietin; megakaryocyte stimulating factor; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand; thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand]

products gene name :

[TPO]

other gene names :

[THPO; THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1; MGDF; ML; MGDF]

uniprot entry name :

TPO_HUMAN

reactivity :

Human

sequence length :

353

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Tnip3 Concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TNIP3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

914226

ncbi acc num :

AAB33390.1

ncbi mol weight :

33,604 Da

ncbi pathways :

Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Hemostasis Pathway (106028); Platelet Aggregation (Plug Formation) Pathway (106053); Platelet Activation, Signaling And Aggregation Pathway (106034)

ncbi summary :

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]

uniprot summary :

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Cell cycle regulation; Secreted; Cytokine. Chromosomal Location of Human Ortholog: 3q27. Cellular Component: extracellular space. Molecular Function: growth factor activity; hormone activity; cytokine activity. Biological Process: positive regulation of protein kinase B signaling cascade; platelet activation; cell proliferation; multicellular organismal development; positive regulation of megakaryocyte differentiation; myeloid cell differentiation; positive regulation of protein amino acid phosphorylation; blood coagulation. Disease: Thrombocythemia 1

size1 :

48-Strip-Wells

price1 :

250 USD

size2 :

96-Strip-Wells

price2 :

365

size3 :

5x96-Strip-Wells

price3 :

1480

size4 :

10x96-Strip-Wells

price4 :

2620