Human heterogeneous nuclear ribonucleoprotein compies / anti-RA33-antibody (hnRNP / RA33) ELISA Kit | MyBioSource MBS264450 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human heterogeneous nuclear ribonucleoprotein compies / anti-RA33-antibody (hnRNP / RA33) ELISA Kit

catalog :

MBS264450

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS264450

products type :

ELISA Kit

products full name :

Human heterogeneous nuclear ribonucleoprotein compies / anti-RA33-antibody (hnRNP / RA33) ELISA Kit

products short name :

heterogeneous nuclear ribonucleoprotein compies / anti-RA33-antibody (hnRNP / RA33)

other names :

Heterogeneous nuclear ribonucleoproteins A2/B1; Heterogeneous nuclear ribonucleoproteins A2/B1; heterogeneous nuclear ribonucleoproteins A2/B1; hnRNP A2 / hnRNP B1; nuclear ribonucleoprotein particle A2 protein; heterogeneous nuclear ribonucleoprotein A2/B1

products gene name :

hnRNP / RA33

other gene names :

HNRNPA2B1; HNRNPA2B1; RNPA2; HNRPA2; HNRPB1; SNRPB1; HNRNPA2; HNRNPB1; IBMPFD2; HNRPA2B1; HNRPA2B1; hnRNP A2/B1

uniprot entry name :

ROA2_HUMAN

reactivity :

Human

sequence length :

353

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant hnRNP/RA33 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human hnRNP/RA33 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

133257

ncbi acc num :

P22626.2

ncbi mol weight :

36,006 Da

ncbi pathways :

Gene Expression Pathway (105937); Processing Of Capped Intron-Containing Pre-mRNA Pathway (160950); MRNA Splicing Pathway (105951); MRNA Splicing - Major Pathway (105952); MRNA Processing Pathway (198843)

ncbi summary :

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

hnRNP A2/B1: Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Identified in the spliceosome C complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with IGF2BP1. 2 isoforms of the human protein are produced by alternative splicing. Protein type: RNA-binding; Spliceosome; RNA splicing. Chromosomal Location of Human Ortholog: 7p15. Cellular Component: nucleoplasm; spliceosome; membrane; cytoplasm; ribonucleoprotein complex; nucleus. Molecular Function: protein binding; single-stranded telomeric DNA binding; RNA binding; nucleotide binding. Biological Process: nuclear mRNA splicing, via spliceosome; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; negative regulation of transcription from RNA polymerase II promoter; mRNA processing; RNA transport. Disease: Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 2

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage