Mouse pituitary adenylate cyclase activating polypeptide (PACAP) ELISA Kit | MyBioSource MBS264350 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse pituitary adenylate cyclase activating polypeptide (PACAP) ELISA Kit

catalog :

MBS264350

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS264350

products type :

ELISA Kit

products full name :

Mouse pituitary adenylate cyclase activating polypeptide (PACAP) ELISA Kit

products short name :

pituitary adenylate cyclase activating polypeptide (PACAP)

other names :

pituitary adenylate cyclase activating polypeptide; Pituitary adenylate cyclase-activating polypeptide; pituitary adenylate cyclase-activating polypeptide; prepro-PACAP; adenylate cyclase activating polypeptide 1 (pituitary); PRP-48

products gene name :

PACAP

other gene names :

ADCYAP1; ADCYAP1; PACAP; PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38

uniprot entry name :

PACA_HUMAN

reactivity :

Mouse

sequence length :

176

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PACP concentration. Assay Type: Sandwich. Detection Range: 50 ng/ml-0.78 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse PACP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

245806

ncbi acc num :

AAB21470.1

ncbi mol weight :

18,835 Da

ncbi pathways :

Activation Of TRKA Receptors Pathway (106456); Class B/2 (Secretin Family Receptors) Pathway (106378); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Glucagon-type Ligand Receptors Pathway (106380); Insulin Secretion Pathway (777534); NGF Signalling Via TRKA From The Plasma Membrane Pathway (106459); NGF-independant TRKA Activation Pathway (106458); SIDS Susceptibility Pathways (198901)

ncbi summary :

This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]

uniprot summary :

ADCYAP1: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Belongs to the glucagon family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 18p11. Cellular Component: extracellular space; extracellular region; terminal button. Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; receptor signaling protein activity; pituitary adenylate cyclase-activating peptide receptor binding; pituitary adenylate cyclase activating polypeptide activity; receptor binding. Biological Process: behavioral fear response; nerve growth factor receptor signaling pathway; negative regulation of potassium ion transport; female pregnancy; sensory perception of pain; positive regulation of vasodilation; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell cycle; histamine secretion; elevation of cytosolic calcium ion concentration; cAMP-mediated signaling; regulation of protein localization; ovarian follicle development; cell-cell signaling; neuropeptide signaling pathway; positive regulation of cell proliferation; neurite development; adenylate cyclase activation; negative regulation of acute inflammatory response to antigenic stimulus; positive regulation of interleukin-6 production; response to starvation; ATP metabolic process; positive regulation of growth hormone secretion; regulation of postsynaptic membrane potential; response to ethanol; pituitary gland development; positive regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of acute inflammatory response to non-antigenic stimulus; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of G-protein coupled receptor protein signaling pathway

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage