ELISA/assay

Human cyclooxygenase-2 (COX-2) ELISA Kit

MBS264304

48-Strip-Wells

275 USD

ELISA Kit

Human cyclooxygenase-2 (COX-2) ELISA Kit

cyclooxygenase-2 (COX-2)

cyclooxygenase-2, partial; Prostaglandin G/H synthase 2; prostaglandin G/H synthase 2; PHS II; PGH synthase 2; cyclooxygenase 2b; prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2

COX-2

PTGS2; PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS; COX2; COX-2; PGH synthase 2; PGHS-2

PGH2_HUMAN

Human

22

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant COX-2 concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/ml

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Human COX-2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

9230775

AAF85977.1

68,996 Da

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); C-MYB Transcription Factor Network Pathway (138073); C20 Prostanoid Biosynthesis Pathway (545299); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Eicosanoid Synthesis Pathway (198888)

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]

COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family. Protein type: Lipid Metabolism - arachidonic acid; Oxidoreductase; EC 1.14.99.1. Chromosomal Location of Human Ortholog: 1q25.2-q25.3. Cellular Component: endoplasmic reticulum membrane; neuron projection; protein complex; cytoplasm; caveola; nucleus. Molecular Function: prostaglandin-endoperoxide synthase activity; peroxidase activity; protein homodimerization activity; enzyme binding; arachidonate 15-lipoxygenase activity; metal ion binding; heme binding; lipid binding. Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; positive regulation of smooth muscle cell proliferation; response to lithium ion; response to glucocorticoid stimulus; positive regulation of NF-kappaB import into nucleus; response to lipopolysaccharide; decidualization; prostaglandin biosynthetic process; sensory perception of pain; positive regulation of synaptic transmission, glutamatergic; negative regulation of synaptic transmission, dopaminergic; response to estradiol stimulus; bone mineralization; negative regulation of cell cycle; negative regulation of cell proliferation; response to vitamin D; lipoxygenase pathway; nicotinamide metabolic process; negative regulation of smooth muscle contraction; regulation of blood pressure; arachidonic acid metabolic process; positive regulation of smooth muscle contraction; angiogenesis; inflammatory response; water-soluble vitamin metabolic process; response to fructose stimulus; positive regulation of prostaglandin biosynthetic process; response to drug; negative regulation of calcium ion transport; vitamin metabolic process; cyclooxygenase pathway; positive regulation of synaptic plasticity; learning; memory; hair cycle; prostaglandin metabolic process; ovulation; response to manganese ion; NAD metabolic process; regulation of inflammatory response; brown fat cell differentiation; positive regulation of vasoconstriction; positive regulation of fever; response to oxidative stress; cell motility; embryo implantation

48-Strip-Wells

275 USD

96-Strip-Wells

420