ELISA/assay

Rat Osteocalcin (OT) ELISA Kit

MBS264256

48-Strip-Wells

290 USD

ELISA Kit

Rat Osteocalcin (OT) ELISA Kit

Osteocalcin (OT)

osteocalcin preproprotein; Osteocalcin; osteocalcin; bone Gla protein; gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate (gla) protein; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein

OT

BGLAP; BGLAP; OC; BGP; OCN; BGP

OSTCN_HUMAN

Rat

100

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant OT concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat OT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

40316933

NP_954642.1

NM_199173.4

10,963 Da

Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); FGF Signaling Pathway (137989); Glucocorticoid Receptor Regulatory Network Pathway (138014); Interleukin-11 Signaling Pathway (698753); M Phase Pathway (105812); Mitotic Anaphase Pathway (105818); Mitotic Metaphase And Anaphase Pathway (730317); Mitotic Prometaphase Pathway (105815); Notch-mediated HES/HEY Network Pathway (169347)

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family. Protein type: Secreted, signal peptide; Cell development/differentiation; Secreted. Chromosomal Location of Human Ortholog: 1q22. Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; cytoplasm; dendrite; perikaryon. Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity. Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; osteoblast differentiation; odontogenesis; regulation of osteoclast differentiation; response to vitamin D; response to ethanol; response to vitamin K; response to zinc ion; response to estrogen stimulus; response to mechanical stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; regulation of bone mineralization; skeletal development

48-Strip-Wells

290 USD

96-Strip-Wells

450