Human Telomerase (TE) ELISA Kit | MyBioSource MBS264236 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Telomerase (TE) ELISA Kit

catalog :

MBS264236

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS264236

products type :

ELISA Kit

products full name :

Human Telomerase (TE) ELISA Kit

products short name :

Telomerase (TE)

other names :

telomerase reverse transcriptase isoform 1; Telomerase reverse transcriptase; telomerase reverse transcriptase; telomerase catalytic subunit; telomerase-associated protein 2; telomerase reverse transcriptase; HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2; TP2

products gene name :

other gene names :

TERT; TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1; EST2; TCS1; TRT; TP2

uniprot entry name :

TERT_HUMAN

reactivity :

Human

sequence length :

1132

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TE concentration. Assay Type: Sandwich. Detection Range: 50 ng/ml-0.78 ng/ml. Sensitivity: 0.08 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TE monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

109633031

ncbi acc num :

NP_937983.2

ncbi gb acc num :

NM_198253.2

ncbi mol weight :

88,965 Da

ncbi pathways :

Cell Cycle Pathway (530733); Chromosome Maintenance Pathway (161048); Disease Pathway (530764); Extension Of Telomeres Pathway (106541); HIF-1-alpha Transcription Factor Network Pathway (138045); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); IL2 Signaling Events Mediated By PI3K Pathway (137938); Id Signaling Pathway (198871); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1016209)

ncbi summary :

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008]

uniprot summary :

TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined. Protein type: Nucleolus; EC 2.7.7.49; DNA-binding; Transferase. Chromosomal Location of Human Ortholog: 5p15.33. Cellular Component: nucleoplasm; PML body; chromosome, telomeric region; nuclear telomere cap complex; telomerase holoenzyme complex; nucleolus. Molecular Function: telomerase activity; protein binding; protein homodimerization activity; RNA-directed RNA polymerase activity; DNA binding; metal ion binding; telomeric DNA binding; RNA-directed DNA polymerase activity; telomeric template RNA reverse transcriptase activity; nucleotidyltransferase activity; tRNA binding. Biological Process: mitochondrion organization and biogenesis; DNA strand elongation; RNA-dependent DNA replication; positive regulation of nitric-oxide synthase activity; telomere maintenance via telomerase; age-dependent telomere shortening; RNA interference, production of siRNA; telomere maintenance; positive regulation of Wnt receptor signaling pathway. Disease: Pulmonary Fibrosis, Idiopathic; Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage