catalog number :
MBS264117
products type :
ELISA Kit
products full name :
Mouse Bone-specific Alkphase B (ALP-B) ELISA Kit
products short name :
Bone-specific Alkphase B (ALP-B)
other names :
Asparaginase like 1; Isoaspartyl peptidase/L-asparaginase; isoaspartyl peptidase/L-asparaginase; beta-aspartyl-peptidase; isoaspartyl dipeptidase; L-asparagine amidohydrolase; asparaginase-like 1 protein; asparaginase-like protein 1; asparaginase like 1; Asparaginase-like protein 1; Beta-aspartyl-peptidase; Isoaspartyl dipeptidase; L-asparagine amidohydrolaseCleaved into the following 2 chains:Isoaspartyl peptidase/L-asparaginase alpha chain; Isoaspartyl peptidase/L-asparaginase beta chain
products gene name :
ALP-B
other gene names :
ASRGL1; ASRGL1; ALP; ALP1; CRASH; ALP; CRASH
uniprot entry name :
ASGL1_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ALP-B concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse ALP-B monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAH21295.3
ncbi mol weight :
18,981 Da
ncbi pathways :
Asparagine Degradation I Pathway (142343)
uniprot summary :
ASRGL1: Acts in asparagine catabolism. May be involved in astroglial production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Belongs to the Ntn-hydrolase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Other Amino Acids Metabolism - cyanoamino acid; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate; Hydrolase; EC 3.5.1.1; EC 3.4.19.5. Chromosomal Location of Human Ortholog: 11q12.3. Cellular Component: cytoplasm; nucleus. Molecular Function: asparaginase activity; N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity; beta-aspartyl-peptidase activity. Biological Process: asparagine catabolic process via L-aspartate; proteolysis