catalog number :
MBS264018
products type :
ELISA Kit
products full name :
Guinea pig Interferon Alpha (IFN-alpha) ELISA Kit
products short name :
Interferon Alpha (IFN-alpha)
products name syn :
Guinea pig Interferon Alpha (IFN-a) ELISA Kit
other names :
interferon-alpha; Interferon omega-1; interferon omega-1; interferon alpha-II-1; IFN-omega 1, interferon omega-1; interferon, omega 1; Interferon alpha-II-1
products gene name :
IFN-alpha
other gene names :
IFNW1; IFNW1
uniprot entry name :
IFNW1_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Guinea pig serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IFN-? concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.
other info2 :
Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%
products description :
Princple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Guinea pig IFN-? monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAA52724.1
ncbi mol weight :
22,319 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); RIG-I-like Receptor Signaling Pathway (117292); RIG-I-like Receptor Signaling Pathway (116128)
uniprot summary :
IFNW1: Belongs to the alpha/beta interferon family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 9p22. Cellular Component: extracellular space. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; response to virus; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; natural killer cell activation during immune response; innate immune response; cell cycle arrest; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein