Human Dopamine (DA) ELISA Kit | MyBioSource MBS264001 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Dopamine (DA) ELISA Kit

catalog :

MBS264001

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS264001

products type :

ELISA Kit

products full name :

Human Dopamine (DA) ELISA Kit

products short name :

Dopamine (DA)

other names :

D(1B) dopamine receptor; D(1B) dopamine receptor; D(1B) dopamine receptor; D(1B) dopamine receptor; dopamine D5 receptor; dopamine receptor D1B; d(5) dopamine receptor; D1beta dopamine receptor; dopamine receptor D5; D(5) dopamine receptor; D1beta dopamine receptor; Dopamine D5 receptor

products gene name :

other gene names :

DRD5; DRD5; DBDR; DRD1B; DRD1L2; DRD1B; DRD1L2

uniprot entry name :

DRD5_HUMAN

reactivity :

Human

sequence length :

477

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human s erum, plasma or cell culture supernatant and organizations in the natural and recombinant DA concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: Up to 12 pg/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human DA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

4503391

ncbi acc num :

NP_000789.1

ncbi gb acc num :

NM_000798.4

ncbi mol weight :

52,951 Da

ncbi pathways :

Amine Ligand-binding Receptors Pathway (106363); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Dopamine Receptors Pathway (106366); Dopaminergic Synapse Pathway (469199); Dopaminergic Synapse Pathway (469185); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020)

ncbi summary :

This gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2. [provided by RefSeq, Jul 2008]

uniprot summary :

DRD5: Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Defects in DRD5 are a cause of benign essential blepharospasm (BEB). BEB is a primary focal dystonia affecting the orbicularis oculi muscles. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. BEB usually begins in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. Patients have normal eyes. The visual disturbance is due solely to the forced closure of the eyelids. In severe cases, this can lead to functional blindness. Belongs to the G-protein coupled receptor 1 family. Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1. Chromosomal Location of Human Ortholog: 4p16.1. Cellular Component: brush border membrane; integral to plasma membrane; plasma membrane. Molecular Function: dopamine receptor activity; dopamine binding; dopamine D1 receptor-like receptor activity. Biological Process: synaptic transmission, dopaminergic; wound healing; adenylate cyclase activation; response to amphetamine; transmission of nerve impulse; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; response to cocaine; G-protein signaling, adenylate cyclase activating pathway; cellular calcium ion homeostasis; synaptic transmission; regulation of female receptivity; regulation of systemic arterial blood pressure by vasopressin; sensitization; negative regulation of blood pressure; positive regulation of adenylate cyclase activity; dopamine receptor, phospholipase C activating pathway; negative regulation of NAD(P)H oxidase activity; associative learning; dopamine receptor, adenylate cyclase activating pathway. Disease: Attention Deficit-hyperactivity Disorder; Blepharospasm, Benign Essential

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage