product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human epidermal keratin (EK) ELISA Kit
catalog :
MBS263921
quantity :
48-Strip-Wells
price :
275 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS263921
products type :
ELISA Kit
products full name :
Human epidermal keratin (EK) ELISA Kit
products short name :
epidermal keratin (EK)
other names :
epidermal type I keratin, partial; Keratin, type I cytoskeletal 14; keratin, type I cytoskeletal 14; CK-14; keratin-14; cytokeratin 14; cytokeratin-14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14; Cytokeratin-14; CK-14; Keratin-14; K14
products gene name :
EK
other gene names :
KRT14; KRT14; K14; NFJ; CK14; EBS3; EBS4; CK-14; K14
uniprot entry name :
K1C14_HUMAN
reactivity :
Human
sequence length :
157
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant EK concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human EK monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi gi num :
177139
ncbi acc num :
AAA96689.1
ncbi mol weight :
51,561 Da
ncbi pathways :
Corticotropin-releasing Hormone Pathway (920957); Glucocorticoid Receptor Regulatory Network Pathway (138014)
ncbi summary :
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
uniprot summary :
K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21.2. Cellular Component: keratin filament; cytoplasm; intermediate filament; cytosol; nucleus. Molecular Function: protein binding; structural constituent of cytoskeleton. Biological Process: hemidesmosome assembly; epidermis development; epithelial cell differentiation; response to zinc ion; intermediate filament bundle assembly; response to ionizing radiation; hair cycle; aging. Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type
size1 :
48-Strip-Wells
price1 :
275 USD
size2 :
96-Strip-Wells
price2 :
420
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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