catalog number :
MBS263889
products type :
ELISA Kit
products full name :
Human Albumin (ALB) ELISA Kit
products short name :
Albumin (ALB)
other names :
albumin; Serum albumin; serum albumin; albumin (32 AA); albumin (AA 34); growth-inhibiting protein 20; cell growth inhibiting protein 42; albumin
other gene names :
ALB; ALB; FDAH; ANALBA; PRO0883; PRO0903; PRO1341; GIG20; GIG42; PRO0903; PRO1708; PRO2044; PRO2619; PRO2675; UNQ696/PRO1341
uniprot entry name :
ALBU_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant ALB concentration. Assay Type: Sandwich. Detection Range: 200 ug/ml-3.12 ug/ml. Sensitivity: 0.6 ug/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ALB monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAA98797.1
ncbi mol weight :
47,360 Da
ncbi pathways :
Bile Acid And Bile Salt Metabolism Pathway (106144); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); HDL-mediated Lipid Transport Pathway (106158); Hemostasis Pathway (106028); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Platelet Activation, Signaling And Aggregation Pathway (106034)
ncbi summary :
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]
uniprot summary :
albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Carrier; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular space; protein complex; extracellular region; basement membrane; nucleus. Molecular Function: antioxidant activity; toxin binding; protein binding; copper ion binding; enzyme binding; DNA binding; zinc ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding. Biological Process: receptor-mediated endocytosis; platelet activation; sodium-independent organic anion transport; bile acid metabolic process; maintenance of mitochondrion localization; lipoprotein metabolic process; hemolysis by symbiont of host red blood cells; cellular response to starvation; response to mercury ion; bile acid and bile salt transport; response to organic substance; retinal homeostasis; platelet degranulation; transport; negative regulation of programmed cell death; blood coagulation; positive regulation of circadian sleep/wake cycle, non-REM sleep; transmembrane transport; response to nutrient; negative regulation of apoptosis. Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic